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Key Documents

SRP3237

Sigma-Aldrich

TRAIL from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):

Apo2 Ligand, TL2, TNF-related apoptosis-inducing Ligand, TNFSF10

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

40.0-60.0 ng/mL ED50

mol wt

20.0 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P50592

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... TNFSF10(22035)

General description

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a cytotoxic protein. Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.

Biochem/physiol Actions

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apoptosis is achieved through binding to two death-signaling receptors, death receptor 4 and 5 (DR4 and 5). These receptors belong to the tumor necrosis factor receptor (TNFR) superfamily of transmembrane proteins and contain a cytoplasmic "death domain", which activates the cell′s apoptotic machinery.

Sequence

MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Death receptor 5, a new member of the TNFR family, and DR4 induce FADD-dependent apoptosis and activate the NF-kappaB pathway.
Chaudhary PM
Immunity, 7(6), 821-830 (1997)
S A Marsters et al.
Recent progress in hormone research, 54, 225-234 (1999-11-05)
Apo2 ligand (Apo2L, also called TRAIL) is a member of the tumor necrosis factor (TNF) cytokine family. The closest homolog of Apo2L is CD95 (Fas/Apo1) ligand, to which it has 24% amino acid sequence identity. Similar to CD95L, Apo2L activates
Apoptosis by death factor.
S Nagata
Cell, 88(3), 355-365 (1997-02-07)
Abdul G Hameed et al.
The Journal of experimental medicine, 209(11), 1919-1935 (2012-10-17)
Pulmonary arterial hypertension (PAH) is a life-threatening disease characterized by the progressive narrowing and occlusion of small pulmonary arteries. Current therapies fail to fully reverse this vascular remodeling. Identifying key pathways in disease pathogenesis is therefore required for the development
G Pan et al.
Science (New York, N.Y.), 277(5327), 815-818 (1997-08-08)
TRAIL, also called Apo2L, is a cytotoxic protein that induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types. An antagonist decoy receptor (designated as
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