SRP2082

WT-1 (+KTS) human

recombinant, expressed in insect cells, ≥60% (SDS-PAGE)

• UNSPSC Code: 12352200
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in insect cells
• assay: ≥60% (SDS-PAGE)
• form: frozen liquid
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 150 μg/mL
• color: clear colorless
• NCBI accession no.: NM_024425
• UniProt accession no.: P19544
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... WT1(7490)

Description

Biochem/physiol Actions

WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable