SRP0286
Sphingosine kinase 2 Active from mouse
recombinant, expressed in baculovirus, ≥70% (SDS-PAGE)
Synonym(s):
SK2, SPHK2, SPK 2
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
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recombinant
expressed in baculovirus
assay
≥70% (SDS-PAGE)
form
aqueous solution
specific activity
≥2 units/μg
mol wt
68 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
mouse ... SPHK2(56848)
General description
Mouse Sphingosine kinase 2 ( GenBank Accession No. NM_203280), full length with N-terminal His tag, MW = 68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Sphingosine kinase 2 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 2 has been used in a study to assess blood–brain barrier protection in hypoxic preconditioning-induced cerebral ischemic tolerance. Sphingosine kinase 2 has also been used in a study to investigate sensitization of human colon cancer cells to sodium butyrate-induced apoptosis.
Unit Definition
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Certificates of Analysis (COA)
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Mithun R Raje et al.
Bioorganic & medicinal chemistry, 20(1), 183-194 (2011-12-06)
Sphingosine kinase (SphK) has emerged as an attractive target for cancer therapeutics due to its role in cell survival. SphK phosphorylates sphingosine to form sphingosine 1-phosphate (S1P), which has been implicated in cancer growth and survival. SphK exists as two
Min Xiao et al.
Experimental cell research, 318(1), 43-52 (2011-10-26)
Sphingosine kinases (SphKs) have been recognized as important proteins regulating cell proliferation and apoptosis. Of the two isoforms of SphK (SphK1 and SphK2), little is known about the functions of SphK2. Sodium butyrate (NaBT) has been established as a promising
Bradley K Wacker et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 32(6), 1014-1023 (2012-02-09)
Protection of the blood-brain barrier (BBB) is correlated with improved outcome in stroke. Sphingosine kinase (SphK)-directed production of sphingosine-1-phosphate, which we previously documented as being vital to preconditioning-induced stroke protection, mediates peripheral vascular integrity via junctional protein regulation. We used
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