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SRP0142

Sigma-Aldrich

PRMT3 human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

Synonym(s):

Arginine methyltransferase 3, HMT1 hnRNP methyltransferase-like 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like 3

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥60% (SDS-PAGE)

form

aqueous solution

mol wt

86 kDa

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_005788

UniProt accession no.

O60678

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRMT3(10196)

General description

Human PRMT3, GenBank Accession No. NM_005788, amino acids 2-end, with N-terminal GST tag, MW = 86 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificates of Analysis (COA)

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Mamta Verma et al.
Communications biology, 4(1), 109-109 (2021-01-27)
Protein arginine methyltransferase 3 (PRMT3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins. However, very little is known about the interaction partners of PRMT3 and their functional outcomes. Using yeast-two hybrid screening, we identified

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