Human PRMT1, GenBank Accession No. NM_001536, amino acids 2- end, with N-terminal GST tag, MW = 68 kDa, expressed in E. coli expression system.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.