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SRE0008

Sigma-Aldrich

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 20-60 units/mg protein (modified Warburg-Christian)

Synonym(s):

GALT, UDP glucose:α-D-galactose-1-phosphate uridyltransferase

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About This Item

CAS Number:
9016-11-9
Enzyme Commission number:
2.7.7.12 (BRENDA, IUBMB)
MDL number:
MFCD00131120
UNSPSC Code:
12352204
NACRES:
NA.32

form

lyophilized powder

specific activity

20-60 units/mg protein (modified Warburg-Christian)

composition

Protein, 15-35%

application(s)

diagnostic assay manufacturing

foreign activity

6-phosphogluconate dehydrogenase ≤0.5%
UDP glucose pyrophosphorylase and galactokinase ≤0.2%

shipped in

wet ice

storage temp.

−20°C

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Biochem/physiol Actions

Galactose-1-phosphate uridyltransferase catalyzes the conversion of UDP-glucose to galactose-1-phosphate and galactose-1-phosphate to UDP-glucose.

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from UDP-glucose, galactose 1-phosphate and NADP+ per min at pH 8.7 at 25 °C as detected by a coupled system using phosphoglucomutase.

Physical form

Contains buffer salts as citrate and reduced glutathione

Analysis Note

Protein determined by modified Warburg-Christian

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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