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SCA20

Supelco

Sucrose Assay Kit

sufficient for 20 assays

Synonym(s):

Sucrose Test Kit

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About This Item

EC Number:
UNSPSC Code:
12164500
NACRES:
NA.84

usage

sufficient for 20 assays

analyte chemical class(es)

sugars (sucrose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

General description

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

Kit Components Only

Product No.
Description

  • glucose assay reagent 50 mL

  • sucrose assay reagent 1 mL/vial

  • sucrose standard 500 mg

Storage Class

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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