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SAB4600388

Sigma-Aldrich

Anti-Mouse IgG (H+L), F(ab′)2 fragment, CF488A antibody produced in goat

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

NACRES:
NA.46

Quality Level

biological source

goat

conjugate

CF 488A conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

~2 mg/mL

technique(s)

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1-10 μg/mL
western blot: suitable

fluorescence

λex 490 nm; λem 515 nm

storage temp.

−20°C

General description

Digestion of IgG by papain results in generation of fragment antigen binding (Fab), comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallizable (fc), comprising of the H chain constant region. IgG1 is the most abundant antibody and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection. IgG antibody has enormous therapeutic potential and the Fc region is used for the development of therapeutic antibodies.
This product is prepared by labeling high quality F(ab′)2 fragment of goat anti-mouse IgG (H+L) with the green fluorescent dye CF488A. CF488A is a superior alternative to the traditional green fluorescent dye FITC (or fluorescein) owing to its exceptional brightness and photostability. CF488A is comparably bright and photostable to Alexa Fluor 488. However, antibody conjugates prepared from CF488A are more specific than those prepared from Alexa Fluor 488 because CF488A, being minimally charged, does not significantly change the isoelectric points of antibodies as Alexa Fluor 488 does.

Specificity

Binds with whole molecule mouse IgG and the light chains of other mouse immunoglobulins.

Immunogen

mouse IgG(H+L)

Application

Anti-Mouse IgG (H+L), F(ab?)2 fragment, CF488A antibody produced in goat has been used in the detection of CF488 in human cell lines using confocal fluorescence microscopy and in vinculin cytoskeleton using confocal immunofluorescence imaging.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Preparation Note

Protect from light. The antibody solution should be gently mixed before use.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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