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SAB4200808

Sigma-Aldrich

Anti-Myosin Light Chain Kinase antibody, Mouse monoclonal

clone K36, purified from hybridoma cell culture

Synonym(s):

Kinase-related protein (KRP), MLCK, Myosin light chain kinase, Smooth muscle, Telokin, smMLCK

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

K36, monoclonal

form

buffered aqueous solution

mol wt

~160 kDa

species reactivity

bovine, human, mouse, chicken, pig, turkey

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using chicken gizzard extract

isotype

IgG2b

UniProt accession no.

Q15746

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Myosin Light Chain Kinase (MLCK) also known as MYLK is a Ca2+/calmodulin dependent myosin light chain phosphorylating agent. This enzyme plays a major role in the phosphorylation of the regulatory light chains of myosin which are essential for the shortening and tension development of smooth muscle cells resulting in smooth muscle contraction. Myosin light chain kinase are widely expressed in many different tissues and cells of eukaryote species. There are two genes mylk1 and mylk2 encoding the MLCK protein, mylk2 is exclusively expressed in skeletal muscle cells.

Immunogen

Purified chicken gizzard myosin light chain kinase.

Application

Monoclonal Anti-Myosin Light Chain Kinase recognizes the myosin light chain kinase of smooth muscle and from non-muscle cells such as cultured fibroblasts. Reactivity has been observed with myosin light chain kinase from chicken, turkey, bovine, human, mouseand pig origin. The antibody is recommended to use in various immunological techniques, including Immunoblotting (~160kDa), Immunohistochemistry and immunofluorescence.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Myosin Light Chain Kinase: A Potential Target for Treatment of Inflammatory Diseases.
Xiong, et al.
Frontiers in Pharmacology, 8, 292-292 (2017)
Renaud Léguillette et al.
American journal of respiratory and critical care medicine, 179(3), 194-204 (2008-11-18)
Airway smooth muscle (SM) of patients with asthma exhibits a greater velocity of shortening (Vmax) than that of normal subjects, and this is thought to contribute to airway hyperresponsiveness. A greater Vmax can result from increased myosin activation. This has
Jeffrey B Wyckoff et al.
Current biology : CB, 16(15), 1515-1523 (2006-08-08)
Tumor cells invading three-dimensional matrices need to remodel the extracellular matrix (ECM) in their path. Many studies have focused on the role of extracellular proteases; however, cells with amoeboid or rounded morphologies are able to invade even when these enzymes
S Topouzis et al.
Developmental biology, 178(2), 430-445 (1996-09-15)
Lineage analysis studies in the avian embryo have identified two types of smooth muscle cells (SMCs) in the tunica media of large elastic arteries; one that originates within the cardiac neural crest and is ectoderm in origin (Ect) and another
Zoe M Goeckeler et al.
The Journal of biological chemistry, 280(38), 33083-33095 (2005-08-02)
This study determined the effects of increased intracellular cAMP and cAMP-dependent protein kinase activation on endothelial cell basal and thrombin-induced isometric tension development. Elevation of cAMP and maximal cAMP-dependent protein kinase activation induced by 10 microm forskolin, 40 microm 3-isobutyl-1-methylxanthine
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