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Key Documents

SAB4200770

Sigma-Aldrich

Anti-Human IgG4-Peroxidase antibody, Mouse monoclonal

clone HP-6025, purified from hybridoma cell culture

Synonym(s):

Anti-Human immunoglobulin G4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HP-6025, monoclonal

form

lyophilized powder

species reactivity

human

concentration

~2 mg/mL

technique(s)

ELISA: 1:80,000-1:160,000 using 1 μg/mL human IgG4 for coating

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Human IgG consists of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. It constitutes approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. Only IgG1 and IgG3 are capable of adhering to mononuclear phagocytes while IgG2 and IgG4 auto-antibodies are not associated with disorders such as hemolytic anemia.Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiextasia. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and thus may assist in the diagnosis of various disorders. For example IgG subclass expression pattern in malaria patients reveals positive association of IgG4 levels and malaria antigens but not with patients age.Monoclonal Anti-Human IgG4 specifically recognizes Human IgG4. The antibody shows no cross-reactivity with human IgG1, IgG2 and IgG3. The IUIS/WHO study evlauted this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies

Immunogen

Purified human IgG4 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

The antibody is recommended to use in various immunological techniques, including ELISA.

Physical form

Supplied as lyophilized powder. After reconstitution with 0.25 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline

Other Notes

Reconstitute the content of the vial with 0.25 ml of distilled water to a final antibody concentration of ∼2 mg/ml.

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GHS07

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hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
067M4798
51170601

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P Aucouturier et al.
Clinical and experimental immunology, 68(2), 392-396 (1987-05-01)
Serum IgG subclass levels were determined using a competitive indirect immunoenzymatic assay with monoclonal antibodies in 16 patients with ataxia-telangiectasia. Eight children had IgA deficiency, two had IgG and IgA deficiency and six patients showed no immunoglobulin class abnormality. However
A Nasr et al.
Scandinavian journal of immunology, 74(4), 390-396 (2011-06-08)
The anti-malarial IgG immune response during the lengthy and dry season in areas of low malaria transmission as in Eastern Sudan is largely unknown. In this study, ELISA was used for the measurement of pre-existing total IgG and IgG subclasses
J D Isaacs et al.
Clinical and experimental immunology, 106(3), 427-433 (1996-12-01)
It is traditionally held that human IgG4 MoAbs should not deplete target cells in vivo, as this isotype is inactive in a number of in vitro assays that measure effector function. We have previously challenged this dogma, and the current
F W van der Meulen et al.
British journal of haematology, 46(1), 47-56 (1980-09-01)
The purpose of this study was to determine whether quantitative or qualitative factors are of major importance in the destruction of red cells sensitized with incomplete warm autoantibodies of subclass IgG1. To that end, the relative amount of igG1 antibody
R Jefferis et al.
Immunology letters, 10(3-4), 223-252 (1985-01-01)
Seventy-four monoclonal antibodies (McAb) of putative specificity for human IgG (11), the IgG sub-classes (59) or Gm allotypes (4) have been evaluated for reactivity and specificity in eight laboratories employing different assay techniques or protocols. For the IgG, IgG3, IgG4
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