Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

SAB4200716

Sigma-Aldrich

Anti-Vimentin antibody, Mouse monoclonal

enhanced validation

clone VIM-13.2, purified from hybridoma cell culture

Synonym(s):

Anti-F5H288

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

VIM-13.2, monoclonal

form

buffered aqueous solution

mol wt

~58 kDa

species reactivity

mouse, bovine, canine, rat, monkey, human, chicken

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract
immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells
immunohistochemistry: suitable

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... Vim(280955)
chicken ... Vim(420519)
dog ... Vim(477991)
human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)
rhesus monkey ... Vim(705289)

General description

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.

Immunogen

human foreskin fibroblast extract

Application

Anti-Vimentin antibody, Mouse monoclonal has been used in:
  • western blotting
  • indirect immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy
Biskou O, et al.
Testing, 14(1), e0209665-e0209665 (2019)
Morphological and quantitative analysis on alpha-tubulin modifications in glioblastoma cells
Gadau SD
Neuroscience Letters, 687, 111-118 (2018)
Podoplanin regulates mammary stem cell function and tumorigenesis by potentiating Wntbeta-catenin signaling
Bresson L, et al.
Development, 145(4), dev160382-dev160382 (2018)
S Geiger et al.
Oral surgery, oral medicine, and oral pathology, 60(5), 517-523 (1985-11-01)
A 15-year-old boy was referred to the ear, nose, and throat clinic because of a swelling in the upper premolar region. The initial diagnosis of a poorly differentiated soft-tissue sarcoma was made. Further immunohistochemical studies established a definitive diagnosis of
Jui M Dave et al.
Microcirculation (New York, N.Y. : 1994), 21(4), 333-344 (2014-01-07)
Angiogenesis is a multistep process that requires intricate changes in cell shape to generate new blood vessels. IF are a large family of proteins that play an important structural and functional role in forming and regulating the cytoskeleton. Vimentin, a

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service