SAB4200478

Monoclonal Anti-FUS antibody produced in mouse

clone FUS-4, tissue culture supernatant

• Synonym(s): Monoclonal Anti-75 kDa DNA-pairing protein, Monoclonal Anti-ALS6, Monoclonal Anti-FUS1, Monoclonal Anti-HNRNPP2, Monoclonal Anti-POMP75, Monoclonal Anti-TLS, Monoclonal Anti-fused in sarcoma, Monoclonal Anti-fusion gene in myxoid liposarcoma, Monoclonal Anti-heterogeneous nuclear ribonucleoprotein P2, Monoclonal Anti-translocated in liposarcoma protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: FUS-4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: mouse, human, rat
• technique(s): immunohistochemistry: 1:500-1:1000 using formalin-fixed and paraffin embedded rat cerebellum.; indirect immunofluorescence: 1:200-1:400 using using HeLa or HepG2 cells.; western blot: 1:1000-1:2000 using lysates of G361 cells.
• isotype: IgM
• UniProt accession no.: P35637
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FUS(2521) ; mouse ... Fus(233908) ; rat ... Fus(317385)

Description

General description

Monoclonal Anti-FUS (mouse IgM isotype) is derived from the hybridoma FUS-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FUS, conjugated to KLH. Fused in sarcoma (FUS), also known as translocated in liposarcoma (TLS), ribonucleoprotein (RNP)-P2, amyotrophic lateral sclerosis-6 (ALS6), is a RNA/DNA binding protein. FUS is normally located predominantly in the nucleus whereas, pathological FUS inclusions are mostly found in the cytosol of neurons and glia cells.

Immunogen

synthetic peptide corresponding to an internal sequence of human FUS, conjugated to KLH. The corresponding sequence is identical in monkey and differs by 3 amino acids in mouse and rat.

Application

Monoclonal Anti-FUS antibody produced in mouse has been used in various immunochemical techniques including:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

Fused in sarcoma (FUS) plays a vital role in transcription, RNA splicing and transport and is implicated in multiple diseases. Mutations in TAR DNA binding protein 43 (TDP-43) and FUS is associated with the development of amyotrophic lateral sclerosis (ALS) and fronto-temporal lobar degeneration (FLTD) including ubiquitin-positive inclusions (FLTDU). The majority of the FUS mutations has been recognized in C-terminal nuclear localization signal (NLS). FUS has been implicated in a broadening spectrum of neurodegenerative disorders. FUS has been identified as a component of inclusion bodies in patients with Huntington′s disease (HD) and spinocerebellar ataxias (SCA1-3).

Physical form

Culture supernatant solution containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable