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SAB4200344

Sigma-Aldrich

Anti-SMYD3 antibody, Mouse monoclonal

clone SMYD3-2, purified from hybridoma cell culture

Synonym(s):

Anti-KMT3E, Anti-SET and MYND domain containing 3, Anti-ZMYND1, Anti-Zinc finger MYND domain-containing protein 1, Anti-Zinc finger protein, subfamily 3A (MYND domain containing), 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SMYD3-2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~43 kDa

species reactivity

rat, mouse, human

concentration

~1.0 mg/mL

technique(s)

western blot: 5-10 μg/mL using whole extracts of HeLa cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SMYD3(64754)
mouse ... Smyd3(69726)
rat ... Smyd3(498295)

General description

Monoclonal Anti-SMYD3 (mouse IgG1 isotype) is derived from the hybridoma SMYD3-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to a fragment of human SMYD3. SMYD3 belongs to the SMYD family of chromatin regulators. It is a histone methyltransferase, that plays a role in transcriptional regulation as a member of an RNA polymerase complex. SMYD3 contains a SET domain, which is critical for the methylation of histone H3 at Lys4 and a myeloid, Nervy, and DEAF-1 (MYND)-type zinc-finger domain, which is common to developmental proteins.

Immunogen

Fusion protein corresponding to a fragment of human SMYD3.

Application

Monoclonal Anti-SMYD3 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

SMYD3 is important in heart and skeletal muscle development. Overexpression of SMYD3 is observed in hepatocellular cancer, colorectal cancer and breast cancer. SMYD3 is essential for estrogen receptor-regulated gene transcription in the estrogen signaling pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells
Hamamoto R, et al.
Nature Cell Biology, 6(8), 731-731 (2004)
Requirement of histone methyltransferase SMYD3 for estrogen receptor-mediated transcription
Kim H, et al.
The Journal of Biological Chemistry, 284(30), 19867-19877 (2009)
Structural insights into the autoinhibition and posttranslational activation of histone methyltransferase SmyD3
Sirinupong N, et al.
Journal of Molecular Biology, 406(1), 149-159 (2011)

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