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SAB4200274

Sigma-Aldrich

Anti-EIF2C2/Ago2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Eukaryotic translation initiation factor 2C, 2, Anti-I2C2, Argonaute-2, Anti-PAZ Piwi domain protein, Anti-Slicer protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~97 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human EIF2C2/Ago2

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EIF2C2(27161)
mouse ... Eif2c2(239528)
rat ... Eif2c2(59117)

General description

Anti-eukaryotic translation initiation factor 2C 2 (EIF2C2)/protein argonaute-2 (Ago3) is produced in rabbit using as immunogen a synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2 conjugated to KLH. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. Ago subfamily consists of hsAgo1-4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies.

Immunogen

synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Ago2 has endonuclease activity, that cleaves the target RNA in RNAi. Ago2 is regulated at both the transcriptional and posttranslational levels in human breast cancer cell lines. It is implicated in micro-RNA activity and in the tumorigenic progression of breast cancer cell lines.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Exosomes from hepatitis C infected patients transmit HCV infection and contain replication competent viral RNA in complex with Ago2-miR122-HSP90.
Bukong TN, Momen-Heravi F, Kodys K, et al.
PLoS Pathogens, 10(10), e1004424-e1004424 (2014)
Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells
Adams BD, et al.
Endocrinology, 150(1), 14-23 (2008)
Argonaute proteins: mediators of RNA silencing
Peters L and Meister G
Molecular Cell, 26(5), 611-623 (2007)
Terence N Bukong et al.
PLoS pathogens, 10(10), e1004424-e1004424 (2014-10-03)
Antibodies targeting receptor-mediated entry of HCV into hepatocytes confer limited therapeutic benefits. Evidence suggests that exosomes can transfer genetic materials between cells; however, their role in HCV infection remains obscure. Here, we show that exosomes isolated from sera of chronic
The Argonaute protein family
Hock J and Gunter M
Genome Biology, 9(2), 210-210 (2008)

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