Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

View All Documentation

SAB4200151

Sigma-Aldrich

Anti-Drosha antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-ETOHI2, RANSE3L, Anti-RN3, Anti-RNASEN, Anti-RNC, Anti-RNase III, Anti-p241, Anti-ribonuclease type III, nuclear

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 130-160 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells.
western blot: 2-4 μg/mL using HeLa nuclear cell lysates.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RNASEN(29102)

General description

Drosha ribonuclease III (Drosha) is encoded by the gene mapped to human chromosome 5p13.3. It is a double-stranded RNA (dsRNA)-specific RNase III enzyme. The encoded protein contains RNase III domains (RIIIDs) on the top and central domain (CED) at the bottom. In addition, it also contains unique non-canonical zinc-finger motifs.

Application

Anti-Drosha antibody produced in rabbit has been used in western blotting and immunofluorescence.

Biochem/physiol Actions

Drosha ribonuclease III (Drosha) plays a vital role in initiation of microRNA (miRNA) maturation. It catalyzes the cleavage of stem loop structure from the primary microRNA (pri-miRNA).Drosha ribonuclease III (Drosha) plays a vital role in initiation of microRNA (miRNA) maturation. It catalyzes the cleavage of stem loop structure from the primary microRNA (pri-miRNA). Mutation in the gene is associated with development of Epithelial ovarian cancer (EOC). Knockdown of Drosha can enhance the sensitivity of gastric cancer to epirubicin.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A novel role for GSK3? as a modulator of Drosha microprocessor activity and MicroRNA biogenesis
Fletcher CE
Nucleic Acids Research (2016)
[Knockdown of Drosha promotes chemosensitivity of epirubicin for gastric cancer MGC-803 cells].
Xu L
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 32, 1207-1211 (2016)
Niya L Morris et al.
Biochimica et biophysica acta, 1863(10 Pt B), 2645-2653 (2017-04-14)
Ethanol exposure at the time of burn injury is a major contributor to post-burn pathogenesis. Many of the adverse effects associated with ethanol and burn injury are linked to an impaired intestinal barrier. The combined insult causes intestinal inflammation, resulting
Xiaoxiao Zhang et al.
Biochimica et biophysica acta, 1860(12), 1179-1188 (2017-11-08)
microRNAs (miRNAs) are first transcribed as long, primary transcripts, which are then processed by multiple enzymes and proteins to generate the single-stranded, approximately 22-nucleotide (nt)-long mature miRNAs. A critical step in animal miRNA biogenesis is the cleavage of primary miRNA
Structure of Human DROSHA.
Kwon SC
Cell, 164, 81-90 (2016)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service