SAB4200059

Anti-Chimerin β2 antibody, Rat monoclonal

clone HAR 2E3, purified from hybridoma cell culture

• Synonym(s): Anti-ARHGAP3, Anti-BCH, Anti-CHN2, Anti-Chimaerin β2, Anti-GTPase-activating protein, RHO, 3, Anti-RHOGAP3, Anti-Rho-GTPase-activating protein 3, Monoclonal Anti-Chimerin β2 antibody produced in rat
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HAR 2E3, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: human, monkey, rat, mouse, bovine
• packaging: antibody small pack of 25 μL
• technique(s): immunocytochemistry: suitable; western blot: 1.0-2.0 μg/mL using Neuro-2a cell extracts
• isotype: IgG2a
• UniProt accession no.: P52757
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CHN2(1124)

Description

General description

Monoclonal Anti-Chimerin β2 (rat IgG2a isotype) is derived from the hybridoma HAR 2E3 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a fusion protein of full length human Chimerin β2. Chimerin β2 (CHN2) is a member of the chimerin family and encodes a protein with a phorbol-ester/ diacylglycerol (DAG)-type zinc finger containing C1 domain, a Rho-GTPase activating proteins (GAP) domain and an Src homology 2 (SH2) domain. Chimerin β2 gene is mapped to human chromosome 7p14.3.

Specificity

Monoclonal Anti-Chimerin b2 recognizes human, monkey, bovine, mouse and rat Chimerin b2.

Immunogen

fusion protein of full length human Chimerin β2

Application

Monoclonal Anti-Chimerin β2 antibody produced in rat has been used in immunoblotting at a dilution of 1:1000 and immunocytochemistry.

Biochem/physiol Actions

Chimerin β2 protein (CHN2) is a high affinity receptor for the phorbol esters and diacylglycerol (DAG). Binding of DAG induces translocation of the protein from the cytosol to the Golgi apparatus membrane. It has guanosine triphosphatase GTPase-activating protein activity regulated by phospholipid binding. Decreased expression of CHN2 gene is associated with high-grade gliomas and breast. Deregulation in expression of CHN2 gene is associated with lymphomas, glioblastoma and insulin resistance. Mutations in the CHN2 gene have been associated with schizophrenia in men.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable