SAB3701180

Anti-Mouse IgG2a (γ-chain specific)-Biotin antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• conjugate: biotin conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: mouse
• technique(s): immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. It belongs to the immunoglobulin family and is a widely expressed serum antibody. Disulfide bonds link the two heavy chains, the heavy and light chains and also links residues inside the chains. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. IgG2 is involved in immune responses to bacterial capsular polysaccharide antigens. Its deficiency has been linked to an increased susceptibility to bacterial infections.

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Biotin, Anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA. Typically less than 1% cross reactivity was observed against other Mouse heavy chain isotypes.

Immunogen

Mouse IgG2a heavy chain

Physical properties

Antibody format: IgG

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS09
• signalword: Danger
• hcodes: H302,H311,H411
• pcodes: P280 - P301 + P312 + P330 - P302 + P352 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2
• supp_hazards: EUH032
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable