SAB3500120

Monoclonal Anti-ORAI3 antibody produced in mouse

clone 2H2G9, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Calcium release-activated calcium channel protein 3, Anti-TMEM142C, Anti-Transmembrane protein 142C
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2H2G9, monoclonal
• form: buffered aqueous solution
• mol wt: predicted mol wt 32 kDa
• species reactivity: rat, human
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• UniProt accession no.: Q9BRQ5
• shipped in: dry ice
• storage temp.: −20°C

Description

Immunogen

raised against a 19 amino acid peptide from near the carboxy terminus of human ORAI3.

Features and Benefits

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Target description

Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2 (5). Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.

Physical form

Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable