RAB0326

C-Peptide EIA Kit

for serum, plasma, culture supernatant and cell lysates

• Synonym(s): C-Peptide
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: human, mouse, rat
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; enzyme immunoassay: suitable
• input: sample type plasma; sample type cell lysate; sample type culture supernatant(s); sample type serum
• assay range: inter-assay cv: <15%; intra-assay cv: <10%; sensitivity: 772 pg/mL; standard curve range: 0.1-1000 ng/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... INS(3630)

Description

General description

The C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain.

Immunogen

C-Peptide , synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

C-peptide inhibits endothelial dysfunction under high glucose conditions via inhibition of nuclear factor-κB (NF-κB) pathway. Experimental studies suggest that prolonged dosage of physiological concentration of C-peptide to type 1 diabetes patients minimizes diabetes-induced complications, such as glomerular hypertrophy and mesangial matrix expansion by interrupting transforming growth factor β1 (TGF-β1) and tumor necrosis factor α (TNFα) signaling pathway. C-peptide reduces diabetes-induced glomerular hyperfiltration by contraction of the afferent arteriole, dilation of the efferent arteriole, and by inhibition of tubular reabsorption. C-Peptide also plays a crucial role in renal pathophysiology. C-peptide binds to a cell surface receptor that is likely to be G-protein coupled. This interaction stimulates various cellular functions such as increasing intracellular Ca²⁺ concentration, enhancing PI3-K (phosphoinositide 3-kinase) activity, increased endothelial nitric oxide synthase (eNOS) transcription, and activation of the Na⁺/K⁺ ATPase and mitogen-activated protein kinase (MAPK) signaling pathway.

Kit Components Also Available Separately

• RABHRP3 HRP-Streptavidin

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8A - Combustible corrosive hazardous materials