P8953

Platelet-Derived Growth Factor from porcine platelets

PDGF, lyophilized powder, suitable for cell culture

• Synonym(s): PDGF
• MDL number: MFCD00166903
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: Porcine
• Quality Level: 200
• assay: ≥97% (SDS-PAGE and N-terminal analysis)
• form: lyophilized powder
• potency: 39.00 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: glycoprotein 38 kDa
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• color: white
• UniProt accession no.: P20034
• storage temp.: −20°C
• Gene Information: pig ... PDGFB(100626465)

Description

Application

PDGF elicits multifunctional actions with a variety of cells, including mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts.

Biochem/physiol Actions

The principal mitogen found in mammalian serum, released from platelets during clot formation. Elicits mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. Mitogenic for dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. Appears to interact with transforming growth factor-β1 in accelerating wound healing. However, platelet-derived growth factor (PDGF) may also be pathogenic in arteriosclerosis and neoplasia.

Physical form

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid, pH 2.0.

Analysis Note

The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)