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P6374

Sigma-Aldrich

p53 human

recombinant, expressed in baculovirus infected Sf21 cells

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.25

biological source

human

Quality Level

200

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥90% (SDS-PAGE)

form

aqueous solution

technique(s)

dot blot: suitable

UniProt accession no.

P04637

application(s)

genomic analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TP53(7157)

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General description

p53 protein is composed of an N-terminal domain with a transactivation domain and proline-rich region, a central core-DNA binding domain, and a C-terminal domain with tetramerization and regulatory domains. The TP53 gene which encodes for the p53 protein is mapped on the human chromosome at 17p13.1.

Application

p53 human has been used in dot blot.
Useful for the study of postranslational modification of p53, or protein-protein interaction studies.

Biochem/physiol Actions

In addition, p53 was recently shown to induce differentiation of human embryonic stem cells. The effect is dependent on the DNA binding activity of p53.
p53 gene is highly conserved and expressed in normal tissues. It is the most commonly mutated gene in human cancer and more then 500 gene mutations have been described in various types of malignancies, hematologic as well as solid tumors. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells. Thus, p53 plays a vital role in suppressing the development of cancer.
p53 protein acts as a transcription factor that participates in regulating positively or negatively the expression of several responsive genes. It plays a role in regulating angiogenesis, cellular senescence, autophagy, cell survival, differentiation, and oxidative stress. Mutations in the TP53 gene lead to Li Fraumeni cancer syndrome.

Physical properties

Histidine-tagged, full length human p53

Physical form

Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA, and 1 mM DTT.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Fiamma Mantovani et al.
Cell death and differentiation, 26(2), 199-212 (2018-12-13)
Forty years of research have established that the p53 tumor suppressor provides a major barrier to neoplastic transformation and tumor progression by its unique ability to act as an extremely sensitive collector of stress inputs, and to coordinate a complex
Abhinav K Jain et al.
PLoS biology, 10(2), e1001268-e1001268 (2012-03-06)
Multiple studies show that tumor suppressor p53 is a barrier to dedifferentiation; whether this is strictly due to repression of proliferation remains a subject of debate. Here, we show that p53 plays an active role in promoting differentiation of human
Se Kyung Lee et al.
PloS one, 10(8), e0124658-e0124658 (2015-08-05)
Overexpression of p53 is the most frequent genetic alteration in breast cancer. Recently, many studies have shown that the expression of mutant p53 differs for each subtype of breast cancer and is associated with different prognoses. In this study, we
Alejandra Ward et al.
BMC cancer, 11, 71-71 (2011-02-18)
Hepatocellular carcinoma (HCC), one of the most common cancers world-wide occurs twice as often in men compared to women. Predisposing conditions such as alcoholism, chronic viral hepatitis, aflatoxin B1 ingestion, and cirrhosis all contribute to the development of HCC. We
Brandon S Sheffield et al.
The American journal of surgical pathology, 39(7), 977-982 (2015-01-31)
A variety of immunohistochemical (IHC) stains have been proposed to mark either benign or malignant mesothelial proliferations. Loss of the p16 tumor suppressor (CDKN2A), through homozygous deletions of 9p21, is a good marker of mesotheliomas but lacks sensitivity. Recent reports
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