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P3026

Sigma-Aldrich

Pectolyase from Aspergillus japonicus

greener alternative

lyophilized powder, ≥0.3 units/mg solid

All Photos(5)
Enzyme Commission number:
3.2.1.15 (BRENDA, IUBMB)
MDL number:
MFCD00131813
NACRES:
NA.54

biological source

Aspergillus sp. (A. japonicus)

Quality Level

200

form

lyophilized powder

specific activity

≥0.3 units/mg solid

mol wt

50 kDa

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative category

Enabling

storage temp.

2-8°C

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This Item
8625010065A9857
Pectolyase from Aspergillus japonicus lyophilized powder, ≥0.3 units/mg solid

Sigma-Aldrich

P3026

Pectolyase from Aspergillus japonicus

α-Amylase from Aspergillus oryzae powder, ~1.5 U/mg (~0.2 U acc. to Willstätter)

Sigma-Aldrich

86250

α-Amylase from Aspergillus oryzae

Sigma-Aldrich

Sigma-Aldrich

10065

α-Amylase from Aspergillus oryzae

α-Amylase from Aspergillus oryzae ≥150 units/mg protein (biuret)

Sigma-Aldrich

A9857

α-Amylase from Aspergillus oryzae

biological source

Aspergillus sp. (A. japonicus)

biological source

Aspergillus sp. (A. oryzae)

biological source

-

biological source

-

form

lyophilized powder

form

powder

form

powder

form

powder

specific activity

≥0.3 units/mg solid

specific activity

~1.5 U/mg (~0.2 U acc. to Willstätter)

specific activity

~30 U/mg

specific activity

≥150 units/mg protein (biuret)

mol wt

50 kDa

mol wt

-

mol wt

Mr ~51000

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.

Application

Pectolyase from Aspergillus japonicus has been used:
  • in the lysis of chlorella cells for protoplast generation
  • as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation
  • to induce reorganization of Arabidopsis root cap cell architecture

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Biochem/physiol Actions

Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.
Pectolyase is used for protoplast isolation from plant cells. It also mediates ethylene hormone synthesis.

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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