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P2482

Sigma-Aldrich

Monoclonal Anti-Protein Kinase Bα antibody produced in mouse

clone PKB-175, ascites fluid

Synonym(s):
Anti-Akt1, Anti-PKBα
MDL number:
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PKB-175, monoclonal

mol wt

antigen 56 kDa

contains

15 mM sodium azide

species reactivity

chicken, rat, bovine, mouse, human

technique(s)

indirect ELISA: suitable
microarray: suitable
western blot: 1:5,000 using human MCF-7 cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... AKT1(207)
mouse ... Akt1(11651)
rat ... Akt1(24185)

General description

Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) (mouse IgG1 isotype) is derived from the PKB-175 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. PKB/Akt is composed of an N-terminal pleckstrin-homology (PH) domain, followed by a catalytic kinase domain and a short C-terminal regulatory domain. This cytoplasmic protein is made up of 480 amino acids. AKT1 is mapped to human chromosome 14q32.
Protein Kinase B (also known as Akt, or RAC-PK) is a protein that belongs to serine/threonine kinases.

Specificity

Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) recognizes PKB isoform α.

Immunogen

Synthetic peptide corresponding to amino acids 461-477 of human PKBα/Akt1, conjugated to KLH. The sequence is identical in mouse, rat, and bovine PKBα/Akt1, highly conserved in PKBβ/Akt2, and diverges in PKBγ.

Application

Monoclonal Anti-Protein Kinase Bα antibody produced in mouse has been used in:
  • western blot
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Protein kinase Bα (PKBα/Akt1) plays a crucial role as a suppressor of apoptotic cell death in different cell types, induced by a variety of stimuli including growth factor withdrawal, loss of cell adhesion and DNA damage. It can protect cerebellar neurons from apoptosis induced by insulin-like growth factor 1 (IGF-1) withdrawal. Akt is the cellular homologue of the viral oncogene v-akt of the AKT-8 acute transforming retrovirus found in rodent T cell lymphoma. It is considered to play an important role in the control of cell cycle, cell proliferation and differentiation . AKT plays a key role in angiogenesis.

Physical form

Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) is supplied as ascites fluid containing 15 mM sodium azide

Storage and Stability

For continuous use, store at 2-8C for upto one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Certificate of Analysis

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Certificate of Origin

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SDS

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