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P1644

Sigma-Aldrich

Percoll®

pH 8.5-9.5 (20 °C)

MDL number:
NACRES:
NA.75

sterility

aseptically filled

Quality Level

application(s)

hematology: suitable
histology: suitable

pH

8.5-9.5 (20 °C)

storage temp.

2-8°C

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General description

Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll consists of colloidal silica particles of 15–30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials.

Application

Percoll® has been used in sperm preparation to study the energy requirements of bovine embryos.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.

Legal Information

Percoll is a registered trademark of Cytiva

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Can you help me calculate gradients for Percoll®, Product P1644?

    The product information sheet (under Documents, above) has an equation for calculating density of a given solution.

  2. When using Product P1644, Percoll®, what density should I use for my particular system?

    The product information sheet (under Documents, above) has a table of densities for different organelles and cell types.

  3. Is there a "best" salt solution to use with Product P1644, Percoll®?

    Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested.  Sucrose is included to prevent aggregation.  You can find detailed preparation instructions on the product information sheet (under Documents, above) for this produc.

  4. Can Percoll®, Product P1644, be autoclaved?

    Percoll can be autoclaved, but this must be done without any salts or sucrose present.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. What is the difference between Percoll® Products P4937 and P1644?

    Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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The Journal of cell biology, 106(4), 1171-1183 (1988-04-01)
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Rajasree Sreedharan et al.
Kidney international, 86(3), 515-524 (2014-05-09)
Inducible heat shock proteins (HSPs), regulated by heat shock factor-1 (HSF-1), protect against renal cell injury in vitro. To determine whether HSPs ameliorate ischemic renal injury in vivo, HSF-1 functional knockout mice (HSF-KO) were compared with wild-type mice following bilateral...
Guilherme D Melo et al.
Scientific reports, 7(1), 8454-8454 (2017-08-18)
Visceral leishmaniasis (VL) is a systemic disease with multifaceted clinical manifestations, including neurological signs, however, the involvement of the nervous system during VL is underestimated. Accordingly, we investigated both brain infection and inflammation in a mouse model of VL. Using...
Development of bovine embryos in vitro as affected by energy substrates.
Rosenkrans Jr C F, et al.
Biology of Reproduction, 49(3), 459-462 (1993)
Noa Bossel Ben-Moshe et al.
Nature communications, 10(1), 3266-3266 (2019-07-25)
Complex interactions between different host immune cell types can determine the outcome of pathogen infections. Advances in single cell RNA-sequencing (scRNA-seq) allow probing of these immune interactions, such as cell-type compositions, which are then interpreted by deconvolution algorithms using bulk...

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