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P0501

Sigma-Aldrich

Phenolphthalein β-D-glucuronide

β-glucuronidase substrate

Synonym(s):
Phenolphthalein glucuronic acid, Phenolphthalein β-D-glucosiduronic acid, Phenolphthalein mono-β-D-glucosiduronic acid
Empirical Formula (Hill Notation):
C26H22O10
CAS Number:
Molecular Weight:
494.45
Beilstein:
68526
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

assay

≥97% (TLC)

form

powder

solubility

H2O: soluble 100 mg/mL

storage temp.

−20°C

SMILES string

O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)C3(OC(=O)c4ccccc34)c5ccc(O)cc5

InChI

1S/C26H22O10/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32/h1-12,19-22,25,27-30H,(H,31,32)/t19-,20-,21+,22-,25+,26?/m0/s1

InChI key

FXJYOZKDDSONLX-XADSOVDISA-N

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Application

β-glucuronidase activity was determined by the amount of phenolphthalein released from 0.01 M henolphthalein β-D-glucuronide.
Phenolphthalein β-D-glucuronide has been used as a substrate for the detection of β-D-glucuronidase activity in fecal bacteria.

Packaging

25, 100, 500 mg in poly bottle

Biochem/physiol Actions

Phenolphthalein β-D-glucuronide is a chromogenic substrate for β-glucuronidase enzyme and the resultant colored product of the reaction is measured at 552 nm.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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