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N5386

Sigma-Aldrich

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

Synonym(s):

Endonuclease micrococcal, MNase, Micrococcal endonuclease

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About This Item

CAS Number:
9013-53-0
Enzyme Commission number:
3.1.31.1 (BRENDA, IUBMB)
EC Number:
232-748-0
MDL number:
MFCD00131731
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Staphylococcus aureus

Quality Level

200

form

powder

specific activity

100-300 units/mg protein

composition

Protein, ≥40% E1%/280 (Balance primarily sodium acetate)

technique(s)

DNA extraction: suitable
DNA purification: suitable

suitability

suitable for molecular biology

application(s)

cell analysis

storage temp.

−20°C

Gene Information

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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Application

Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.
Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Other Notes

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Ho-Ryun Chung et al.
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
Use of micrococcal nuclease to monitor hybridization reactions with DNA.
D L Kacian et al.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
Ashish Kumar Singh et al.
Nature communications, 12(1), 7011-7011 (2021-12-03)
Numerous chromatin remodeling enzymes position nucleosomes in eukaryotic cells. Aside from these factors, transcription, DNA sequence, and statistical positioning of nucleosomes also shape the nucleosome landscape. The precise contributions of these processes remain unclear due to their functional redundancy in
Amnat Phetrungnapha et al.
Fish & shellfish immunology, 34(3), 875-884 (2013-01-22)
RNA interference (RNAi) plays a crucial role as an antiviral defense in several organisms including plants and invertebrates. An understanding of RNAi machineries especially protein components of the RNA-induced silencing complex (RISC) is essential for prior to applying RNAi as
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