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MAK126

Sigma-Aldrich

Bilirubin Assay Kit

sufficient for 180 colorimetric tests

Synonym(s):

Hematoidin Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 180 colorimetric tests

detection method

colorimetric

relevant disease(s)

hematological disorder; gastrointestinal diseases

storage temp.

2-8°C

General description

Bilirubin, also known as hematoidin, is a degradation product formed as a result of heme catabolism in the liver. Bilirubin circulates in the blood stream as either the unconjugated insoluble form (indirect bilirubin) or the soluble glucuronide-conjugated form (direct bilirubin). Conjugated bilirubin moves from the bile canaliculi of the liver to the gall bladder where it is excreted into the small intestine during digestion. High levels of bilirubin can result in jaundice and may indicate liver disease, blood disorders, or blockage of the bile ducts.

Application

Bilirubin Assay Kit has been used to measure the total bilirubin concentration in samples.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of of both total and conjugated bilirubin in serum and plasma samples.

Principle

This assay, based on the Jendrassik-Grof method, utilizes the reaction of bilirubin with diazotized sulfanilic acid resulting in a colorimetric product measured at 530 nm, proportionate to the bilirubin present in the sample. This assay kit measures both total and conjugated bilirubin.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kimberly J Jasmer et al.
Pigment cell & melanoma research, 33(6), 850-868 (2020-06-20)
Biosynthesis and degradation of heme, an iron-bound protoporphyrin molecule utilized by a wide variety of metabolic processes, are tightly regulated. Two closely related enzymes, heme oxygenase 1 (HMOX1) and heme oxygenase 2 (HMOX2), degrade free heme to produce carbon monoxide
Zhi-Ming Ding et al.
Frontiers in pharmacology, 9, 410-410 (2018-05-17)
Understanding of the temporal changes of hepatic lesions in the progression and regression of non-alcoholic steatohepatitis (NASH) is vital to elucidation of the pathogenesis of NASH, and critical to the development of a strategy for NASH pharmacotherapy. There are challenges
Bin Dong et al.
Journal of lipid research, 58(2), 350-363 (2016-12-13)
The farnesoid X receptor (FXR) plays critical roles in plasma cholesterol metabolism, in particular HDL-cholesterol (HDL-C) homeostasis. Obeticholic acid (OCA) is a FXR agonist being developed for treating various chronic liver diseases. Previous studies reported inconsistent effects of OCA on
Progression and regression of hepatic lesions in a mouse model of NASH induced by dietary intervention and its implications in pharmacotherapy.
Ding ZM, et al.
Frontiers in Pharmacology, 9, 410-410 (2018)
Regulation of lipid metabolism by obeticholic acid in hyperlipidemic hamsters.
Dong B,et al.
Journal of Lipid Research, 58(2), 350-363 (2017)

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