M9015

Malachite Green oxalate salt

certified by the Biological Stain Commission

• Synonym(s): N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate, Basic Green 4
• Empirical Formula (Hill Notation): C₂₃H₂₅N₂ · C₂HO₄ · 0.5C₂H₂O₄
• CAS Number: 2437-29-8
• Molecular Weight: 463.50
• Colour Index Number: 42000
• Beilstein/REAXYS Number: 3644550
• EC Number: 219-441-7
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897308
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder or crystals
• composition: Dye content, ≥90%
• color: faint green to very dark green
• mp: 144-150 °C
• solubility: H₂O: 1 mg/mL, blue to very deep blue
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: C[N+](C)=C1C=CC(C=C1)=C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3.[O-]C(C(O)=O)=O
• InChI: 1S/C23H25N2.C2H2O4/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3-1(4)2(5)6/h5-17H,1-4H3;(H,3,4)(H,5,6)/q+1;/p-1
• InChI key: REZFCYOVFCJTDG-UHFFFAOYSA-M

Description

General description

Malachite Green is a basic dye belonging to the triphenylmethane group. It is required in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate.

Application

Malachite Green oxalate salt has been used for phosphate quantification.

Malachite Green oxalate salt has been used:

• in the preparation of Alexander staining solution for cell viability test
• in malachite green-ammonium molybdate assay for determining the adenosine triphosphatase (ATPase) activity of binding immunoglobulin protein (BiP)
• as a hydrophobic ion, to study the label-free molecular transport in the living cell membranes of Escherichia coli in real time by second-harmonic generation (SHG) method

Suitability

Certified for use as a counterstain for paraffin embedded botanical material; as a spore stain for bacteria by the method of Schaeffer and Fulton.

Other Notes

The molecular mass of malachite green oxalate is expressed as either:
927.00 g/mol - the mass of 2 mol malachite green + 2 mol monohydrogen oxalate + 1 mol dihydrogen oxalate, or
463.50 g/mol - the mass of 1 mol malachite green + 1 mol monohydrogen oxalate + 0.5 mol dihydrogen oxalate

Safety Information

• pictograms: GHS05,GHS06,GHS08,GHS09
• signalword: Danger
• hcodes: H301 - H318 - H361fd - H410
• pcodes: P202 - P264 - P273 - P280 - P301 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2
• Storage Class: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable