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M8445

Sigma-Aldrich

Anti-MLH1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-COCA2, Anti-FCC2, Anti-HNPCC, Anti-MGC5172

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 80-85 kDa

species reactivity

human, mouse, rat

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using Jurkat cell lysates

UniProt accession no.

P40692

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MLH1(4292)
mouse ... Mlh1(17350)
rat ... Mlh1(81685)

General description

MLH1 is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BRCA1-associated genome surveillance complex (BASC).
MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is located in chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa.

Specificity

Anti-MLH1 (C-terminal) specifically recognizes human MLH1 (80-85 kDa).

Immunogen

synthetic peptide corresponding to amino acids 591-606 of human MLH1, conjugated to KLH via an N-terminal added cysteine residue. The corresponding peptide sequence is conserved in human, rat, and mouse.

Application

Anti-MLH1 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation

Biochem/physiol Actions

A hereditary mutation in the MLH1 gene is implicated in nonpolyposis colorectal cancer-2.
MutL homolog 1 (MLH1) has been shown to be involved in promoting colorectal carcinogenesis.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lin Chen et al.
Journal of agricultural and food chemistry, 59(6), 2600-2609 (2011-02-19)
Soy protein isolate (SPI) was modified by ultrasound pretreatment (200 W, 400 W, 600 W) and controlled papain hydrolysis, and the emulsifying properties of SPIH (SPI hydrolysates) and USPIH (ultrasound pretreated SPIH) were investigated. Analysis of mean droplet sizes and
Haiyan Chen et al.
Journal of cancer research and clinical oncology, 141(12), 2147-2158 (2015-05-20)
As one of the most essential components of mismatch repair system, MutL homolog 1 (MLH1) plays an increasingly implicated role in initiation and promotion of colorectal carcinogenesis, with germ-line mutations in different loci. However, whether a single genetic variant in
Xiaoqing Chen et al.
Nucleic acids research, 41(20), 9325-9338 (2013-08-14)
Exo1-mediated resection of DNA double-strand break ends generates 3' single-stranded DNA overhangs required for homology-based DNA repair and activation of the ATR-dependent checkpoint. Despite its critical importance in inducing the overall DNA damage response, the mechanisms and regulation of the
Shinichiro Fukuhara et al.
Oncotarget, 5(22), 11297-11307 (2014-12-20)
Mismatch repair (MMR) enzymes have been shown to be deficient in prostate cancer (PCa). MMR can influence the regulation of tumor development in various cancers but their role on PCa has not been investigated. The aim of the present study

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