M6895

Sigma-Aldrich

MEM Vitamin Solution (100×)

sterile-filtered, BioReagent, suitable for cell culture

eCl@ss:
32160406
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

yellow to dark yellow

pH

7.0-8.0

Featured Industry

Agriculture

shipped in

dry ice

storage temp.

−20°C

General description

MEM Vitamin Solution (100×) is a sterile-filtered bioreagent, which is suitable for cell culture.
Synthetic/ basal media used currently contain amino acids, carbohydrates, vitamins and salts. Eagle′s minimum essential medium (MEM) is a modified version of BME (basal medium Eagle′s), which contains increased amounts of each amino acid. Addition of water soluble vitamins such as, inositol to various culture facilitates the hatching of hamster and rabbit blastocysts. Vitamins also influence the metabolism of glucose in sheep and mouse embryos.

Application

MEM Vitamin Solution (100×) has been used in:
  • glial conditioned medium for primary neuronal culture
  • in Luria-Bertani (LB) medium or minimal medium (MM)
  • in private medium (PM = aminoacid-starved medium) for the Aa deprivation experiments

MEM Vitamin Solution (100×) is used for the following applications:
  • In the preparation of DMEM medium (for primary neural cell culture)
  • For the maintenance of cell lines in Eagle′s Minimum Essential Medium (the medium is supplemented with MEM Vitamin Solution along with other components)

Adding supplements of vitamins to media stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

G K Collington et al.
Gut, 42(2), 200-207 (1998-04-16)
The pathophysiology of enteropathogenic Escherichia coli (EPEC) diarrhoea remains uncertain. EPEC adhere to enterocytes and transduce signals which produce a characteristic "attaching and effacing" (A/E) lesion in the brush border membrane. The present in vitro study was designed to determine...
Motoko Tanaka et al.
The Kobe journal of medical sciences, 57(3), E116-E127 (2011-01-01)
Although the viral factors of host adaptation from domestic poultry to humans have been studied several times since the first cases of direct transmission of highly pathogenic avian influenza viruses from domestic poultry to humans were confirmed in 1997, the...
Ken-Ichi Oinuma et al.
Journal of bacteriology, 197(6), 1115-1124 (2015-01-15)
The degradation mechanisms of natural and artificial hydrazides have been elucidated. Here we screened and isolated bacteria that utilize the acylhydrazide 4-hydroxybenzoic acid 1-phenylethylidene hydrazide (HBPH) from soils. Physiological and phylogenetic studies identified one bacterium as Microbacterium sp. strain HM58-2...
Kenneth Verstraete et al.
Nature communications, 8, 14937-14937 (2017-04-04)
The pro-inflammatory cytokine thymic stromal lymphopoietin (TSLP) is pivotal to the pathophysiology of widespread allergic diseases mediated by type 2 helper T cell (Th2) responses, including asthma and atopic dermatitis. The emergence of human TSLP as a clinical target against...
Culture Media, Solutions, and Systems in Human ART
Quinn P
Culture Media, Solutions, and Systems in Human ART. Cambridge University Press (2014)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service