L2769
Lectin from Phaseolus vulgaris (red kidney bean)
Leucoagglutinin PHA-L, lyophilized powder
Synonym(s):
Phaseolus vulgaris agglutinin, PHA
About This Item
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biological source
Phaseolus vulgaris
Quality Level
form
lyophilized powder
potency
erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.)
≤1.0 μg/mL leucoagglutination activity
≤5 μg/mL mitogenic activity
composition
Protein, ≥60% biuret
impurities
salt, free
storage temp.
2-8°C
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General description
Application
- as a positive control in ELISPOT (enzyme-linked immunospot) assay
- to induce Th2 polarization for T-cell differentiation in vitro
- as controls in the lymphocyte proliferation assays
Biochem/physiol Actions
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Preparation Note
Analysis Note
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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