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Key Documents

L0770

Sigma-Aldrich

Lectin from Pisum sativum (pea)

FITC conjugate, lyophilized powder

Synonym(s):

Pisum sativum agglutinin, PSA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

FITC conjugate

Quality Level

200

form

lyophilized powder

potency

<20 μg per mL agglutination activity

composition

Protein, ≥95% biuret

extent of labeling

2-4 mol FITC per mol protein (Lowry).

impurities

salt, free

storage temp.

−20°C

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General description

Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for α-D-glucose and α-D-mannose.

Application

Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns

Biochem/physiol Actions

Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).
PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.

Preparation Note

Conjugates are prepared from affinity purified lectin.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Crypticity and functional distribution of the membrane associated ?-L-fucosidase of human sperm
Venditti JJ, et al.
Molecular Reproduction and Development, 74(6), 758-766 (2007)
Lectin-binding analysis of the biofilm exopolymeric matrix carbohydrate composition of corrosion-aggressive bacteria
Purish LM, et al.
Applied Biochemistry and Microbiology, 49(5), 458-463 (2013)
Amar K Chandra et al.
Environmental toxicology, 32(6), 1823-1835 (2017-02-17)
Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress
In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen
Aires VA, et al.
Theriogenology, 60(2), 269-279 (2003)
Won Young Lee et al.
Asian-Australasian journal of animal sciences, 27(10), 1417-1425 (2014-09-03)
The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters
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