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Key Documents

IAK0111

Sigma-Aldrich

Zymosan A Fluorescent Particles

Red Zymosan conjugate for phagocytosis

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.47

Quality Level

concentration

1 mg/mL

suitability

suitable for fluorescence

storage temp.

2-8°C

General description

Phagocytosis is a cellular process for ingesting and eliminating microorganisms, foreign substances, and apoptotic cells. It is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae) and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays.

Application

Zymosan A fluorescent particles were prepared by conjugating Zymosan A particles with a bright red fluorescent molecular probe. The particles are ready-to-use phagocytosis-inducing particles for fluorescent imaging, microplate reader (Ex/Em = 540/570 nm) and flow cytometric analysis of phagocytosis. We recommend using 5 μL of Zymosan A fluorescent particles per 5 x 105 of viable cells.

Suitability

Suitable for inducing phagocytosis and screening activators and/or inhibitors of phagocytosis and Toll-like receptor ligands (TLR) in mammalian cells.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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