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Key Documents

I2532

Sigma-Aldrich

Monoclonal Anti-Interleukin-7 antibody produced in mouse

clone 7417.111, purified immunoglobulin, lyophilized powder

Synonym(s):

Anti-IL-7

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About This Item

MDL number:
UNSPSC Code:
51111800

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7417.111, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

capture ELISA: suitable
neutralization: suitable
western blot: 1-2 μg/mL

isotype

IgG1

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IL7(3574)

General description

Interleukin-7 (IL-7) is a stromal-cell derived cytokine that is important in the development of adaptive immunity and maintenance of T cells and B cells. IL-7 is produced by bone marrow cells, liver and intestinal epithelia cells, dendritic cells, smooth muscle cells, fibroblasts macrophages and keratinocytes. The effect of IL-7 is mediated by IL-7 receptor α chain and by the subsequent activation of JAK/STAT, PI3K and Src kinases. Important roles of IL-7 include proliferation of lymphoid progenitors, survival and homeostasis of naïve and memory T cells
Monoclonal Anti-Interleukin-7 recognizes recombinant human IL-7 (approximately 17 kDa).

Specificity

The antibody has the ability to neutralize the biological activity of recombinant human IL-7. It may also be used as a capture antibody in a human IL-7 ELISA.

Immunogen

purified recombinant human IL-7, expressed in E. coli.

Application

Anti-Interleukin-7 antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA the working concentration recommended is 1-2 μg/ml. The antibody is also suitable for neutralization assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Modulating T cell Homeostasis with IL-7: Preclinical and Clinical Studies
Capitini CM et al
J. Int. Med., 266, 141-153 (2009)
Charles D Surh et al.
Immunological reviews, 211, 154-163 (2006-07-11)
The pool of memory T cells is regulated by homeostatic mechanisms to persist for prolonged periods at a relatively steady overall size. Recent work has shown that two members of the common gamma chain (gammac) family of cytokines, interleukin-7 (IL-7)
Shoshana D Katzman et al.
Cytokine, 56(1), 116-121 (2011-08-03)
Regulation of the magnitude and quality of immune responses is dependent on the integration of multiple signals which typically operate through positive and negative feedback loops. Cytokines that promote or limit T cell expansion and differentiation are often both present
K S Schluns et al.
Nature immunology, 1(5), 426-432 (2001-03-23)
The naïve and memory T lymphocyte pools are maintained through poorly understood homeostatic mechanisms that may include signaling via cytokine receptors. We show that interleukin-7 (IL-7) plays multiple roles in regulating homeostasis of CD8+ T cells. We found that IL-7
J T Tan et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(15), 8732-8737 (2001-07-12)
In T cell-deficient conditions, naive T cells undergo spontaneous "homeostatic" proliferation in response to contact with self-MHC/peptide ligands. With the aid of an in vitro system, we show here that homeostatic proliferation is also cytokine-dependent. The cytokines IL-4, IL-7, and

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