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GE80-6484-70

SDS-Page Clean-Up Kit

Cytiva 80-6484-70, sufficient for 50 preparations

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About This Item

UNSPSC Code:
41105323
NACRES:
NA.25

usage

sufficient for 50 preparations

feature

wetted part: no

manufacturer/tradename

Cytiva 80-6484-70

packaging

pkg of 50 samples

storage condition

room temperature

General description

SDS-PAGE Clean-Up Kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyze due to the presence of salts or Low Protein concentration.

Application

The SDS-PAGE Clean-Up Kit uses a combination of a precipitant and co-precipitant to quantitatively precipitate the sample Proteins while leaving interfering substances in solution. Detergents, salts, lipids, phenolics, and nucleic acids remain soluble while the Proteins are pelleted by centrifugation. The precipitate is washed further to remove nonProtein contaminants and centrifuged again. The resultant pellet is resuspended, mixed with SDS-PAGE sample buffer, and heated. The sample is then ready for SDS-PAGE. The procedure can be completed in under 2 h with quantitative yield.

Features and Benefits

  • Prepares samples for SDS-PAGE that are otherwise difficult to analyze because of salts or Low Protein concentrations
  • Quantitatively precipitate Proteins while leaving interfering substances in solution
  • Delivers quantitative yield in less than 2 h.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Environment

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Danger

Storage Class

8B - Non-combustible corrosive hazardous materials


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Protocols

The emphasis of this page is on the rules and good practice in sample preparation for Western Blots.

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