GE28-9852-55
Protein G GraviTrap™
Cytiva 28-9852-55, pack of 10 × 1 mL
Synonym(s):
GraviTrap for Protein G, High Performance GraviTrap, Protein G GraviTrap
About This Item
Recommended Products
packaging
pack of 10 × 1 mL
manufacturer/tradename
Cytiva 28-9852-55
storage condition
(20% Ethanol)
bed volume
1 mL
capacity
~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)
storage temp.
2-8°C
General description
Protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
Application
Features and Benefits
- Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
- Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
- High purity and recovery with purity >95% in one step and recovery typically 70% to 80%.
- High binding capacity such as Protein G GraviTrap™ ~ 20 mg human IgG/column
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Protocols
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.
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