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GE28-9321-71

HIS Spintrap™ Kit

Cytiva 28-9321-71, pack of 1 kit

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 1 kit

manufacturer/tradename

Cytiva 28-9321-71

storage condition

(0.15% Kathon CG)
 (20% Ethanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

storage temp.

2-8°C

Related Categories

General description

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Features and Benefits

  • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
  • Ni Sepharose High Performance in prepacked spin columns.
  • Yields up to 750 µg pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

Components

The kit contains:

1 pack - GE28-4013-53 - His Spin Trap™
1 pack - GE11-0034-00 - His Buffer Kit

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

SpinTrap is a trademark of Cytiva

Kit Components Also Available Separately

Product No.
Description
SDS

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H314

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Protocols

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service