GE28-9321-71
HIS Spintrap™ Kit
Cytiva 28-9321-71, pack of 1 kit
About This Item
Recommended Products
packaging
pack of 1 kit
manufacturer/tradename
Cytiva 28-9321-71
storage condition
(0.15% Kathon CG)
(20% Ethanol)
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
storage temp.
2-8°C
Related Categories
General description
Features and Benefits
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose High Performance in prepacked spin columns.
- Yields up to 750 µg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
Components
1 pack - GE28-4013-53 - His Spin Trap™
1 pack - GE11-0034-00 - His Buffer Kit
Storage and Stability
Analysis Note
Legal Information
Kit Components Also Available Separately
- GE11-0034-00His Buffer Kit, Cytiva 11-0034-00SDS
- GE28-4013-53His Spintrap™, Cytiva 28-4013-53SDS
signalword
Danger
hcodes
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
Protocols
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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