GE28-4058-46

HiTrap^® Capto^™ Adhere

Cytiva 28-4058-46, pack of 5 × 5 mL

• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• ligand: N-benzyl-N-methyl ethanolamine
• description: Ion Exchanger Type (value)
• packaging: pack of 5 × 5 mL
• manufacturer/tradename: Cytiva 28-4058-46
• parameter: <20 mL/min flow rate; 42 psi
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: highly cross-linked agarose
• avg. part. size: 75 (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-14(pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.)
• working range: 3-12(pH interval where the medium can be operated without significant change in function.)
• suitability: suitable for bioprocess medium

Description

General description

HiTrap Capto adhere is prepacked with Capto adhere, a multimodal BioProcess ion exchange resin designed to remove contaminats in post-protein A purification. Capto adhere is a strong anion exchanger with multimodal functionality. The multimodal functionality gives a different selectivity compared to traditional anion exchangers. Capto adhere is designed for post-protein A purification of monoclonal antibodies. Removal of leached protein A, aggregates, host cell proteins, nucleic acids, and viruses from monoclonal antibodies is performed in flowthrough mode at which the antibodies pass directly through the column while the contaminants are adsorbed. For optimal performance of Capto adhere, screening/optimization of loading conditions are required.

Application

Protein polishing using Capto^™ Adhere Multimodal chromatography

Features and Benefits

• High capacity and productivity in flowthrough mode.
• Savings in time and operating costs with a two-step chromatographic process.
• Wide operational window of pH and conductivity.
• Prepacked HiTrap columns for easy screening and convenient process development.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

Capto is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids