GE17-6002-44
Immobiline® Drystrip pH 3-10, 24 cm
Cytiva 17-6002-44, pack of 12 strips
About This Item
Recommended Products
form
solid
feature
wetted part: no
packaging
pack of 12 strips
manufacturer/tradename
Cytiva 17-6002-44
color
Colorless
solubility
cold water: soluble (Insoluble in cold and hot water)
shipped in
dry ice
storage temp.
−20°C
General description
Application
The pH 3–11 NL strip covers most Proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the Proteins evenly over the gel length to obtain maximal resolution.
Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of Protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan™ IPGphor 3 IEF system or Multiphor II flatbed system.
Features and Benefits
- Maximize resolution, loading capacity, and reproducibility of first-dimension isoelectric focusing (IEF).
- Choose from a comprehensive range of overlapping Immobiline® DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
- Select the broad pH 3–11 NL gradient Immobiline® DryStrip gel for fast and efficient screening to gain a broad overview of total Protein distribution.
- For more detailed studies or when using greater Protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
- Higher resolution can be obtained using narrow-range (1 pH unit) Immobiline® DryStrip gels.
- Use matching IPG buffers for optimized running conditions.
- Use IPG strips directly after reswelling without further manipulation.
- Barcoded strips for convenient sample and batch tracking.
Storage and Stability
Analysis Note
Legal Information
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 2
Certificates of Analysis (COA)
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Articles
This page describes troubleshooting second-dimension SDS-PAGE with Multiphor™ II Electrophoresis System.
This page describes troubleshooting first-dimension isoelectric focusing using MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit.
Protocols
2-D Clean-Up Kit from GE Healthcare is designed to prepare samples that would otherwise produce poor 2-D results.
This page shows protocols for running 7–24-cm Immobiline DryStrip gels on the Multiphor II Electrophoresis System.
This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.
This protocol shows how to apply samples by cup loading for isoelectric focusing.
Related Content
This page shows how to apply equilibrated Immobiline DryStrip gels
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