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GE17-5195-01

HiTrap® Phenyl High Performance

Cytiva 17-5195-01, pack of 5 × 5 mL

Synonym(s):

HiTrap Phenyl Column, Phenyl High Performance

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

phenyl

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5195-01

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

2-14

working range

3-13

suitability

suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium

General description

HiTrap® Phenyl HP are prepacked Phenyl Sepharose™ High Performance columns for high-resolution hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elutions conditions.

Features and Benefits

  • Provide convenient and fast small-scale protein purifications.
  • Phenyl Sepharose™ High Performance is a well-established aromatic HIC medium, designed for intermediate and polishing steps where high resolution has priority.
  • Flexible design for use with syringes, single laboratory pump, or chromatography system such as AKTA design.
  • High flow rates and high capacities.
  • Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Related product

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Salt Selection and Buffer Preparation

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature

Protocols

Screening for Selectivity of HIC Medium

This section is about the different ways of screening for selectivity of HIC medium from Cytiva, considering both automated and manual

Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service