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Key Documents

GE11-0034-95

HiTrap® MabSelect SuRe

Cytiva 11-0034-95, pack of 5 × 5 mL

Synonym(s):

prepacked columns, MabSelecT columns

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

alkali-stabilized protein A-derived (E. coli)

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 11-0034-95

storage condition

(20% Ehtanol)
(20% Ethanol)

parameter

flow rate
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked agarose

avg. part. size

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-13.7

working range

3-12

capacity

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

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General description

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect SuRe
HiTrap® MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).

HiTrap® MabSelect columns are prepacked with MabSelect a bioprocess medium for capuring of Mabs from large sample volumes.

Features and Benefits

  • Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
  • Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva
MabSelect SuRe is a trademark of Cytiva
SuRe is a trademark of Cytiva

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Flame

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Warning

hcodes

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

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