G2626

L-Glutamic Dehydrogenase from bovine liver

Type II, 50% glycerol solution, ≥35 units/mg protein

• Synonym(s): L-GLDH, L-Glutamate:NAD[P]⁺ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver
• CAS Number: 9029-12-3
• Enzyme Commission number: 1.4.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131461
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine liver
• type: Type II
• form: glycerol solution (50%)
• specific activity: ≥35 units/mg protein
• mol wt: 310-350 kDa
• UniProt accession no.: P00366
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: cow ... GLUD1(281785)

Description

Application

L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.

Biochem/physiol Actions

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:

• It has a reversible concentration-dependent association, producing higher molecular weight forms.
• Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
• Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Unit Definition

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

Physical form

50% glycerol solution

Analysis Note

Protein determined by biuret.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable