G112000
DMT-dG(tac) Phosphoramidite
Synonym(s):
DMT-dG(tac) amidite
About This Item
Recommended Products
type
for DNA synthesis
product line
Proligo Reagents
assay
≥98% (31P-NMR)
≥98.0% (reversed phase HPLC)
λ
conforms (UV/VIS Identity)
nucleoside profile
base: deoxyguanosine
base protecting group: TAC
2' protecting group: none
5' protecting group: DMT
deprotection: fast
storage temp.
-10 to -25°C
SMILES string
COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(NC(=O)COc5ccc(cc5)C(C)(C)C)=Nc34)(c6ccccc6)c7ccc(OC)cc7
InChI
1S/C52H62N7O9P/c1-34(2)59(35(3)4)69(66-29-13-28-53)68-43-30-46(58-33-54-47-48(58)56-50(57-49(47)61)55-45(60)32-64-42-26-16-36(17-27-42)51(5,6)7)67-44(43)31-65-52(37-14-11-10-12-15-37,38-18-22-40(62-8)23-19-38)39-20-24-41(63-9)25-21-39/h10-12,14-27,33-35,43-44,46H,13,29-32H2,1-9H3,(H2,55,56,57,60,61)/t43-,44+,46+,69?/m0/s1
InChI key
MCBSUBQIVLOCIW-YEZUPXOUSA-N
Looking for similar products? Visit Product Comparison Guide
General description
- Deprotection of the TAC group is ultra-fast: complete deprotection inconcentrated ammonia occurs within 15 minutes at 55 °C or two hours atroom temperature
- Compatible with the AMA deprotection reagent (a mixture of ≥25%ammonia in water with 40% aqueous methylamine I/I, v/v)
- Highly soluble in acetonitrile. No need to add co-solvents such asdimethylformamide or methylene chloride
- Suitable for the synthesis of oligomers with base-labile units e.g., dyes andmodifiers, because of less exposure to ammonia and the possibility ofroom temperature deprotection
- No change is required in the reagents commonly used for DNA synthesis,except that Proligo′s Fast Deprotection Cap A solution is used instead ofCap A solution
- The application of dA(tac) minimizes depurination and improves thequality of oligonucleotides
Features and Benefits
- The deprotection of oligonucleotide synthesis products with the AMAreagent is ultra-fast: complete deprotection requires 10 minutes at 65 °C
- Side reactions at C-monomers through transamination are eliminated
- Not compatible with some base-labile modified nucleosides
- dC(tac)-phosphoramidite can directly substitute for dC(bz)-phosphoramidite
- No change is required in the reagents commonly used for DNA synthesis.Acetonitrile is used to dissolve the phosphoramidite. The standard aceticanhydride capping reagent can be employed.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service