G0876
β-Glucuronidase from Helix pomatia
Type H-2, aqueous solution, ≥85,000 units/mL
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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type
Type H-2
form
aqueous solution
specific activity
≥85,000 units/mL
secondary activity
≤7,500 units/mL sulfatase
shipped in
wet ice
storage temp.
2-8°C
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Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Physical form
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
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Description
Pricing
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
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Articles
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
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