F6304

Anti-FOXP2 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-CAGH44, Anti-Forkhead Box P2, Anti-SPCH1, Anti-Speech and Language Disorder 1, Anti-TNRC10, Anti-Trinucleotide Repeat Containing 10
• MDL number: MFCD06411813
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: human, mouse, rat
• technique(s): microarray: suitable; western blot: 1:500-1:1,000 using extracts of Jurkat cells; western blot: 1:500-1:1,000 using mouse heart tissue extract
• UniProt accession no.: O15409
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FOXP2(93986) ; mouse ... Foxp2(114142)

Description

General description

The superfamily of Forkhead transcription factors (FOX) consists of more than 100 members, with orthologues expressed in a variety of species ranging from yeast to man. They are characterized by a common Forkhead (or winged helix) domain, a variant of the helix-turnhelix motif. Forkhead box P2 (FOXP2) transcripts encode for three different isoforms, I, II and III encoding for proteins of 715, 740, and 623 amino acids respectively. FOXP2 has a polyglutamine tract. FOXP1, FOXP2 and FOXP4 contain a complex N-terminal region responsible for transcriptional repression. This region contains various protein-protein interaction motifs conserved in all three proteins, including a zinc finger and a putative leucine zipper.

Immunogen

synthetic peptide corresponding to amino acids 36-53 of human FOXP2, conjugated to KLH via a C-terminal lysine residue. The immunizing sequence is conserved in mouse and rat.

Application

Anti-FOXP2 antibody is suitable for:

• western blot analysis as primary antibody of the embryonic cortex to study the expression of FoxP2 in neural precursors of the embryonic cortex.
• immunohistochemistry of prostate gland cells to determine the expression level of FOXP2 in prostate cancer.
• microarray
• western blot at a dilution of 1:500-1:1,000 using extracts of Jurkat cells and mouse heart tissue extract.

Biochem/physiol Actions

Forkhead box P2 (FOXP2) is the first gene relevant to the human ability to develop language, offering an opportunity to study neural mechanisms from a molecular perspective. Missense and nonsense mutations have been identifying in families with language impairment. Thus, a point mutation led to the isolation to FOXP2, whereas more recently a truncation was discovered in a family with speech and language difficulties as well.

Forkhead transcription factors (FOX) family consists of more than 100 members. It is widely expressed in a variety of species ranging from yeast to man. It has a common Forkhead (or Winged Helix) domain, a variant of the helix-turnhelix motif. It plays a key regulatory role in embryonic development, differentiation, apoptosis and tumorigenesis. Its subfamily P (FOXP1, FOXP2, FOXP3 and FOXP4) has well defined clinical implications in tumor suppression as well as autoimmunity and development. FOXP2 is composed of a polyglutamine tract and a complex N-terminal region responsible for transcriptional repression. The N-terminal region contains various protein-protein interaction motifs including a zinc finger and a putative leucine zipper. It has been reported that FOXP2 acts as a major part of a multiprotein complex that regulates diverse aspects of tissue restricted gene expression during development.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)