F4772
Monoclonal Anti-Cytokeratin Peptide 18−FITC antibody produced in mouse
clone CY-90, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-Cytokeratin Peptide 18
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Recommended Products
biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CY-90, monoclonal
form
buffered aqueous solution
species reactivity
wide range
technique(s)
direct immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded sections of human or animal tissues
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... KRT18(3875) , KRT18(3875)
Looking for similar products? Visit Product Comparison Guide
General description
Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues.
FITC Monoclonal Anti-Cytokeratin Peptide 18 is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the CY-90 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratin 18 (CYK18) is a 45 kDa polypeptide, differentially expressed in various human tissues.
Immunogen
human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Application
Monoclonal Anti-Cytokeratin Peptide 18 FITC antibody produced in mouse has been used in:
- direct immunofluorescent staining
- immunohistochemistry
- double immunofluorescence
- immunocytochemical and immuno?electron microscopic localization of keratins
Monoclonal Anti-Cytokeratin Peptide 18-FITC antibody:
- is suitable for immunohistochemistry to identify renal tubular epithelial cells in the extrarenal cells regeneration during acute renal failure
- is suitable for immunocytochemical and immuno-electron microscopic localisation of keratins in human materno-foetal interaction zone
- may be used for single or double labeling procedures
Biochem/physiol Actions
Cytokeratins also called keratins (Ks) according to the new nomenclature system.These are known to be the major structural proteins of epithelial cells. It is heteropolymeric in nature i.e. in cells more than one keratin protein is always present. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues. It maintains the structural integrity of extra-embryonic membrane epithelia. Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group.
Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. FITC Monoclonal Anti-Cytokeratin Peptide 18 is a chain specific antibody which can facilitate typing of normal, metaplastic and neoplastic cells. Cytokeratin 18 (CYK18) is a cytoskeletal protein associated with apoptosis. CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA with 15 mM sodium azide as a preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Sandeep Gupta et al.
Kidney international, 62(4), 1285-1290 (2002-09-18)
Recovery of renal function following acute tubular necrosis (ATN) is dependent on the replacement of necrotic tubular cells with functional tubular epithelium. The source of these new tubular cells is thought to be resident renal tubular cells. The discovery of
C Lampron et al.
The Journal of biological chemistry, 262(10), 4893-4898 (1987-04-05)
Differentiation of F9 embryonal carcinoma cells by retinoic acid treatment results in extraembryonic endoderm-like cells. The effects of this process on the protein composition of the intermediate filaments were studied by two-dimensional gel electrophoresis and by immunoblotting. By this approach
Immunofluorescence confocal laser scanning microscopy and immuno-electron microscopic identification of keratins in human materno-foetal interaction zone
Ahenkorah J, et al.
Journal of Cellular and Molecular Medicine, 13(4), 735-748 (2009)
Cytokeratin 18 is expressed on the hepatocyte plasma membrane surface and interacts with thrombin-antithrombin complexes
Wells MJ, et al.
The Journal of Biological Chemistry, 272(45), 28574-28581 (1997)
Kalipso Halkidou et al.
The Prostate, 59(2), 177-189 (2004-03-26)
Histone deacetylase 1 (HDAC1) is a co-repressor involved in differentiation and proliferation control. It is upregulated in malignant compared to benign tissue, and targets a number of transcription factors including p53. By immunohistochemistry, HDAC1 protein expression was investigated in human
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service