E9530

Erythropoietin from mouse

recombinant, expressed in NSO cells, ≥90% (SDS-PAGE), lyophilized powder, suitable for cell culture

• Synonym(s): EPO
• MDL number: MFCD01867534
• NACRES: NA.32

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in NSO cells
• assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• potency: 1.030 ng/mL
• mol wt: ~36 kDa by SDS-PAGE; predicted mol wt 18.6 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P07321
• storage temp.: −20°C
• Gene Information: mouse ... Epo(13856)

Description

General description

EPO (erythropoietin) gene is localized to human chromosome 7q22. This protein is predominantly produced by the cortex cells of kidney, as well as a variety of tissues including female reproductive organs. It is a glycoprotein, which has a molecular weight of 34kDa. It is produced as a 193-amino acid precursor protein, which is cleaved to produce a secreted protein of 166-amino acid.

Biochem/physiol Actions

EPO (erythropoietin) plays an essential role in the growth and differentiation of erythrocyte progenitor cells. It is involved in the pathogenesis of myomatous erythrocytosis syndrome, which is characterized by erythrocytosis and myomatous uterus. It acts as a ligand for a homodimer of EPO-receptor (Epo-R), which is localized to the surface of immature erythroid cells. This interaction leads to inhibition of apoptosis and facilitation of cell proliferation and differentiation to mature erythrocytes.

Erythropoietin is a glycoprotein that is the principal regulator of red blood cell growth and differentiation.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

The biological acitivity is measured by its ability to stimulate cell proliferation using TF-1 cell line.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves