MilliporeSigma
All Photos(1)

E9530

Sigma-Aldrich

Erythropoietin from mouse

recombinant, expressed in NSO cells, ≥90% (SDS-PAGE), lyophilized powder, suitable for cell culture

Synonym(s):
EPO
MDL number:
NACRES:
NA.32

biological source

mouse

Quality Level

recombinant

expressed in NSO cells

assay

≥90% (SDS-PAGE)

form

lyophilized powder

potency

1.030 ng/mL

mol wt

~36 kDa by SDS-PAGE
predicted mol wt 18.6 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Epo(13856)

General description

EPO (erythropoietin) gene is localized to human chromosome 7q22. This protein is predominantly produced by the cortex cells of kidney, as well as a variety of tissues including female reproductive organs. It is a glycoprotein, which has a molecular weight of 34kDa. It is produced as a 193-amino acid precursor protein, which is cleaved to produce a secreted protein of 166-amino acid.

Biochem/physiol Actions

EPO (erythropoietin) plays an essential role in the growth and differentiation of erythrocyte progenitor cells. It is involved in the pathogenesis of myomatous erythrocytosis syndrome, which is characterized by erythrocytosis and myomatous uterus. It acts as a ligand for a homodimer of EPO-receptor (Epo-R), which is localized to the surface of immature erythroid cells. This interaction leads to inhibition of apoptosis and facilitation of cell proliferation and differentiation to mature erythrocytes.
Erythropoietin is a glycoprotein that is the principal regulator of red blood cell growth and differentiation.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

The biological acitivity is measured by its ability to stimulate cell proliferation using TF-1 cell line.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service