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Extract-N-Amp PCR ReadyMix

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue


Quality Level


sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications


dNTPs included





shipped in

wet ice

storage temp.


General description

Extract-N-Amp PCR ReadyMix is a specially formulated hot start PCR master mix for direct PCR amplification from the extract.
PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, if additional PCR reactions are required, supplemental PCR ReadyMix may be needed.


Extract-N-Amp PCR ReadyMix has been used for the following applications:
  • Genomic DNA extraction
  • Fungal DNA extraction and amplification by polymerase chain reaction (PCR) reactions
  • Direct PCR amplification
  • Genotyping
  • Real-Time PCR


12, 125 mL in poly bottle

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Melinda Belisle et al.
Microbial ecology, 63(4), 711-718 (2011-11-15)
Microfungi that inhabit floral nectar offer unique opportunities for the study of microbial distribution and the role that dispersal limitation may play in generating distribution patterns. Flowers are well-replicated habitat islands, among which the microbes disperse via pollinators. This metapopulation
Huirong Gao et al.
Frontiers in plant science, 11, 535-535 (2020-05-21)
Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing hybrids with multiple traits is expensive, time-consuming, and complex. Here we report using CRISPR-Cas9 to generate a complex
Muhammad Iqbal et al.
Genome, 50(5), 511-516 (2007-07-07)
Vernalization response (Vrn) genes play a major role in determining the flowering/maturity times of spring-sown wheat. We characterized a representative set of 40 western Canadian adapted spring wheat cultivars/lines for 3 Vrn loci. The 40 genotypes were screened, along with
Beika Zhu et al.
Marine drugs, 17(2) (2019-01-27)
Cell cycle reentry is a unified mechanism shared by several neurodegenerative diseases, including Alzheimer's disease (AD) and Ataxia Telangiectasia (A-T). This phenotype is often related to neuroinflammation in the central nervous system. To mimic brain inflammation in vitro, we adopted
Aifang Cheng et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(2), E292-E301 (2017-12-28)
ATM (ataxia-telangiectasia mutated) and ATR (ATM and Rad3-related) are large PI3 kinases whose human mutations result in complex syndromes that include a compromised DNA damage response (DDR) and prominent nervous system phenotypes. Both proteins are nuclear-localized in keeping with their


Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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