ERGIC (ER-Golgi intermediate compartment) is related to type I membrane marker protein ERGIC-53 and rat homolog is referred as (p58) . ERGIC is a dynamic membrane system made up of tubulo vesicular clusters near to ER exit site which facilitates transport of protein from ER to Golgi. Anti-ERGIC-53/ (p58) antibody can be used as a primary antibody (diluted 1: 125) in fluorescence microscopy. It may also be used for western blotting. Rabbit anti-ERGIC-53/ (p58) antibodies react specifically with ERGIC-53/ (p58) of rat, mouse and human.
synthetic peptide corresponding to amino acids 158-170 of rat p58 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and human.
Anti-ERGIC-53/ (p58) antibody can be used in immunoblotting and immunofluorescence.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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