D4551

Sigma-Aldrich

N,N-Dimethylformamide

for molecular biology, ≥99%

Synonym(s):
DMF, NSC 5356
Linear Formula:
HCON(CH3)2
CAS Number:
Molecular Weight:
73.09
Beilstein/REAXYS Number:
605365
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

Quality Level

grade

for molecular biology

vapor density

2.5 (vs air)

vapor pressure

2.7 mmHg ( 20 °C)

assay

≥99%

form

liquid

autoignition temp.

833 °F

expl. lim.

15.2 %

impurities

≤0.5% water
water

refractive index

n20/D 1.430 (lit.)

bp

153 °C (lit.)

mp

−61 °C (lit.)

density

0.944 g/mL (lit.)

suitability

suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.

storage temp.

room temp

SMILES string

[H]C(=O)N(C)C

InChI

1S/C3H7NO/c1-4(2)3-5/h3H,1-2H3

InChI key

ZMXDDKWLCZADIW-UHFFFAOYSA-N

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General description

N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications.

Application

N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays. It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.
Solvent for many hydrophobic organic compounds.

Packaging

250, 500 mL in glass bottle

Signal Word

Danger

hazcat

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

storage_class_code

3 - Flammable liquids

WGK Germany

WGK 2

Flash Point(F)

135.5 °F - closed cup

Flash Point(C)

57.5 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Lauren Friedman et al.
Anti-cancer drugs, 20(6), 444-449 (2009-04-23)
Curcumin, a yellow pigment and the active component of turmeric, has been shown to protect against carcinogenesis and prevent tumor development in several types of cancer. However, its low bioavailability and potency prevent it from being effective in most chemotherapeutic...
M Kanauchi et al.
Journal of food science, 73(6), C458-C463 (2009-02-26)
For food industry production processes and other uses, a mold that produces high levels of feruloyl esterase was obtained from laboratory mold collections and other sources. It was Aspergillus awanmori G-2 that produces high levels of feruloyl esterase. The feruloyl...
Ruiqin Wu et al.
Food microbiology, 84, 103236-103236 (2019-08-20)
Hepatitis A virus (HAV) continues to be the leading cause of viral hepatitis. HAV outbreaks have been linked to the consumption of milk, but methods for HAV detection in milk are very limited. We developed a method to concentrate HAV...
Takaki Yamauchi et al.
Plant physiology, 169(1), 180-193 (2015-06-04)
In rice (Oryza sativa) roots, lysigenous aerenchyma, which is created by programmed cell death and lysis of cortical cells, is constitutively formed under aerobic conditions, and its formation is further induced under oxygen-deficient conditions. Ethylene is involved in the induction...
Jixian Xu et al.
Nature communications, 6, 7081-7081 (2015-05-09)
Solution-processed planar perovskite devices are highly desirable in a wide variety of optoelectronic applications; however, they are prone to hysteresis and current instabilities. Here we report the first perovskite-PCBM hybrid solid with significantly reduced hysteresis and recombination loss achieved in...
Protocols
cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers.
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